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- 一些缓冲液为什么要加EDTA? - 知乎
比如mcllvaine缓冲液,Tris缓冲液。。甚至醋酸铵溶液这种也加EDTA。。它的用处是什么?为什么要在溶液里…
- Chelation therapy for heart disease: Does it work? - Mayo Clinic
Chelation therapy has long been used as a treatment for mercury and lead poisoning And in the past, some people received chelation therapy to treat heart disease and stroke But it isn't a proven treatment for these conditions Chelation therapy also can cause serious side effects when used as a heart disease treatment
- Lupus - Symptoms causes - Mayo Clinic
Lupus is a disease that occurs when your body's immune system attacks your own tissues and organs (autoimmune disease) Inflammation caused by lupus can affect many different body systems — including your joints, skin, kidneys, blood cells, brain, heart and lungs Lupus can be difficult to diagnose because its signs and symptoms often mimic those of other ailments The most distinctive sign
- Coronary artery disease - Diagnosis and treatment - Mayo Clinic
Coronary artery disease FAQs Mayo Clinic cardiologist Stephen Kopecky, M D , answers the most frequently asked questions about coronary artery disease (CAD)
- 一文读懂硅基负极材料 - 知乎
PAA-CMC 交联粘结剂、 PAA-PVA交联粘结剂、 PAA-PANI 交联粘结剂、 EDTA-PAA 粘结剂等在三维共价交联聚合物的探索性尝试,使复合粘结剂与硅表面形成多点相互作用, 有效防止硅颗粒的脱离, 进一步提升电池循环稳定性,是新型粘接剂的进一步研究方向。
- 铬离子与乙二胺四乙酸二钠不易配位是啥原因? - 知乎
这些多羟基配合物不仅与EDTA反应慢,且可能与EDTA不能形成严格的1比1形式的配合物。 所以需要先加入已知量的过量EDTA,将溶液煮沸,待反应完全,再返滴定。
- 请问DPBS溶液和PBS溶液有什么区别吗? - 知乎
DPBS(Dulbecco's Phosphate-Buffered Saline,即杜氏磷酸盐缓冲液),是用于短期维持细胞活性的一种平衡盐溶液。在有限时间内维持离体细胞结构和生理学上的完整性。pH 为 7 2-7 4,与人体 pH 相近,溶液渗透压与人体血液等渗,不含 Ca2+、Mg2+。适用于各种细胞培养应用,例如解离前清洗细胞、运输细胞或
- 洗脱得到的 His 标签蛋白样品杂带较多、纯度不高,请问各位科研大佬们有什么好的建议吗? - 知乎
可以考虑以下可能原因与解决方法 : (1)His 标签蛋白被部分降解:添加蛋白酶抑制剂 ( 慎用 EDTA);保持蛋白样品在低温下操作;缩短实验操作的时间,例如使用crude 系列预装柱省略离心澄清的步骤; (2)杂蛋白对于金属离子也有较强的结合:优化结合、洗脱条件 ( 咪唑浓度、NaCl 浓度、pH 条件等
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